医学生社会实践论文范文10篇

【关键词】Wnt

ExpressionandbiologicalactivityofmouseWnt3ageneinNIH3T3cells

【Abstract】AIM:ToexpressrecombinantmouseWnt3asignalproteinwithbiologicalactivity.METHODS:Therecombinanteukaryoticexpressionplasmid,pSecTag2/HygroBWnt3a,wastransfectedintoNIH3T3cellsbyliposome,thentheexpressedproteinwasdetectedbyWesternBlot.ThesaturationdensityandthecapabilityofsuppressingapoptosisinWnt3a/NIH3T3cellswereevaluated.RESULTS:TheWnt3asignalproteinwasstablyexpressedinWnt3a/NIH3T3cells.ThesaturationdensityandthecapabilityofsuppressingapoptosisinWnt3a/NIH3T3cellsweremarkedlyincreased.CONCLOUSION:WesuccessfullyconstructtherecombinantWnt3asignalproteinwithbiologicalactivity.

【Keywords】Wnt3a;eukaryoticexpression;contactinhibition;apoptosis

【摘要】目的：表达具备生物活性的重组小鼠Wnt3a信号蛋白.方法：应用脂质体转染试剂将重组真核表达载体pSecTag2/HygroBWnt3a转染并筛选稳定表达的NIH3T3细胞，WesternBlot鉴定重组Wnt3a蛋白的表达，并对Wnt3a/NIH3T3细胞的融合密度及抗凋亡能力给予检测.结果：Wnt3a信号蛋白在Wnt3a/NIH3T3细胞中获得稳定表达，Wnt3a信号蛋白能够明显提高NIH3T3细胞的融合密度及抗凋亡能力.结论：在NIH3T3细胞中表达的重组Wnt3a信号蛋白具备生物活性.

【关键词】Wnt3a；真核表达；接触抑制；细胞凋亡

【关键词】,细胞增殖;,,乳腺癌;,辛基酚；细胞周期蛋白D1;细胞周期蛋白质依赖激酶类

EffectsofoctylphenoloncellcycleandcyclinexpressionofMDAMB231breastcancercells

【Abstract】AIM:Toobservetheeffectsofoctylphenol(OP)oncellcycleandcyclinexpressionofMDAMB231breastcancercellsandtoprobethemolecularmechanismsofOPinhibitingMDAMB231breastcancercellproliferation.METHODS:Thechangesofcellproliferation,cellcycle,cyclinD1mRNA,cyclinD3mRNA,CDK2mRNA,CDK4mRNAandcyclinD1proteinexpressionsofMDAMB231cellstreatedwithOPwereobservedbyusingMTTtest,flowcytometry,immunocytochemistryandRTPCR.RESULTS:WhenMDAMB231breastcancercellsweretreatedwith4or8μmol/LOPfor48h,thecellinhibitionrateswere(17.13±4.1)%,(40.25±8.7)%,andthefluorescencevaluesofcyclinD1were55.1±10.2,41.4±11.2,significantlydecreasedascomparedwithcontrolgroup(89.9±11.2,P<0.05).WhenMDAMB231breastcancercellsweretreatedwith8μmol/LOPfor24and72h,thecellratiosintheG0/G1phasewere(67.0±17.6)%and(44.4±11.9)%，significantlyincreasedascomparedwithcontrolgroup［(46.6±12.8)%at24h,(15.3±3.1)%at72h,P<0.05］.OPinhibitedthemRNAexpressionsofcyclinD1,cyclinD3,CDK2andCDK4andtheproteinexpressionofcyclinD1.CONCLUSION：OPcaninhibittheproliferationofMDAMB231breastcancercells,whichmayberelatedtodownregulatingtheexpressionsofcyclinD1,cyclinD3,CDK2andCDK4mRNAandcyclinD1protein.

【Keywords】cellproliferation;breastneoplasms;octylphenol;cyclinD1；cylindependentkinases

【摘要】目的:观察辛基酚(OP)对细胞周期及周期蛋白表达的影响，探讨OP抑制MDAMB231乳腺癌细胞增殖的可能分子机制.方法：以MTT试验、流式细胞分析、免疫细胞化学和RTPCR等方法观察OP对MDAMB231乳腺癌细胞增殖、细胞周期、CDK2，CDK4，cyclinD1和cyclinD3表达的影响.结果：4,8μmol/LOP作用MDAMB231乳腺癌细胞48h时，其抑制率为(17.1±4.1)%,(40.3±8.7)%，cyclinD1表达量荧光值为55.1±10.2，41.4±11.2，比对照组(89.9±11.2)明显降低(P<0.05).8μmol/LOP作用MDAMB231乳腺癌细胞24h和72h，G0/G1期细胞为(67.0±17.6)%和(44.4±11.9)%，G0/G1期细胞比对照组［24h，(46.6±12.8)%；72h，(15.3±3.1)%］明显增高(P<0.05).OP导致MDAMB231乳腺癌细胞中CDK2，CDK4，cyclinD1和cyclinD3mRNA的表达降低，且cyclinD1蛋白的表达也降低.结论：OP能抑制MDAMB231乳腺癌细胞增殖，其机制可能与OP能降低乳腺癌细胞中CDK2，CDK4，cyclinD1和cyclinD3mRNA及其蛋白的表达有关.

【关键词】细胞增殖;乳腺癌;辛基酚；细胞周期蛋白D1;细胞周期蛋白质依赖激酶类

【关键词】结直肠肿瘤；低氧诱导因子1α；VHL基因；缺氧诱导因子抑制因子1

EffectofVHLmRNAandFIH1mRNAexpressionsincolorectalcarcinomaonHIF1αproteinlevel

【Abstract】AIM:TodetectmRNAexpressionsofVHLandFIH1incolorectalcancer,andexploretheireffectonHIF1αproteinlevel.METHODS:SemiquantitativeRTPCRassaywasusedtodetecttheexpressionsofVHLmRNA,FIH1mRNAin42patientswithcolorectalcancer;ExpressionofHIF1αproteinwasmeasuredbySPimmunohistochemistry;Spearmanrankcorrelationwasusedtoanalyzethecorrelationbetweenthem.RESULTS:mRNAlevelsofVHLandFIH1incolorectalcancerweresignificantlylowerthanthoseinadjacenttissue（t=-10.14,P=0.00；t=-15.26,P=0.00）；BothexpressionsdecreasedasDukesstageincreased,andtheexpressionsinDukesA+BstageweresignificantlyhigherthanthoseinDukesC+Dstage（t=2.84,P=0.01；t=-5.13,P=0.00）；Theirexpressionshadnorelationshipwithtumorlocationandgender.In42casesofcolorectalcancer,positiverateofHIF1αproteinwas69.1%(29/42),theexpressioninDukesC+Dstage(80.0%,24/30)wassignificantlyhigherthanthatinDukesA+Bstage(41.7%,5/12),(t=5.89,P=0.02);itwasnegativeinadjacenttissue;SpearmanrankcorrelationdisplayedthattheexpressionsofVHLmRNAandFIH1mRNAwerenegativelycorrelatedwiththeHIF1αproteinlevel(rs=-0.97,P=0.01;rs=-0.66,P=0.01).CONCLUSION:TheoverexpressionofHIF1αmayplayanimportantroleinthedevelopmentandmatastasisofcolorectalcancer；ThedecreaseofVHLmRNAandFIH1mRNAexpressionsiscloselycorrelatedwiththeincreaseofHIF1αproteinlevelincolorectalcancer.

【Keywords】colorectalneoplasms;HIF1α;VHL;FIH1

【摘要】目的：检测大肠癌中VHLmRNA，FIH1mRNA以及HIF1α蛋白的表达,探讨VHL，FIH1对HIF1α蛋白水平的影响.方法：采用RTPCR技术检测42例大肠癌组织和相应的癌旁正常粘膜组织中VHLmRNA，FIH1mRNA的表达，采用免疫组化SP法检测HIF1α蛋白在大肠癌和癌旁正常组织中的表达，Spearman相关关系检验分析其之间的相关性.结果：大肠癌组织VHLmRNA和FIH1mRNA的表达水平显著低于癌旁正常组织（t=-10.14,P=0.00；t=-15.26,P=0.00）；二者的表达水平随Dukes分期而降低，DukesA+B期显著高于DukesC+D期（t=2.84,P=0.01；t=-5.13,P=0.00）；它们的表达水平与与肿瘤部位、性别无关.大肠癌组织HIF1α蛋白表达阳性率为69.1%(29/42),在癌旁正常组织中均为阴性；DukesC+D期(80.0%,24/30)显著高于DukesA+B期(41.7%,5/12),(t=5.89,P=0.02)；Spearman相关分析显示，HIF1α表达水平与VHLmRNA，FIH1mRNA的表达水平显著负相关（rs=-0.97,P=0.01，rs=-0.66,P=0.01）.结论：HIF1α的过表达在大肠癌的发生发展、浸润转移过程中起着重要作用，大肠癌组织中VHL以及FIH1的水平下降与HIF1α蛋白水平的升高之间存在密切相关.

【关键词】结直肠肿瘤；低氧诱导因子1α；VHL基因；缺氧诱导因子抑制因子1

【关键词】皮肤肿瘤；免疫组织化学；明胶酶B；抗原CD34；微血管密度

ExpressionofMMP9inskinsquamouscellcarcinomaanditssignificanceinmicroangiogenesis

【Abstract】AIM:TodetecttheroleandthesignificanceofMMP9inmicroangiogenesisinskinsquamouscellcarcinoma(SCC)andtoobservetherelationbetweentheexpressionofMMP9andmicrovesseldensity(MVD)inSCC.METHODS：ImmunohistochemicalSPstainingwasusedtoexamineMMP9expressionandthechangeofMVDunderlightmicroscopein45casesofSCC,19casesofBowendisease,and10casesofnormalskin.RESULTS：TheexpressionofMMP9inSCCgroupwassignificantlyhigherthanthatinBowendiseasegroup（P<0.05）,whilethelatterwasalsosignificantlyhigherthanthatinnormalskingroup（P=0.028，P<0.05）.ThelevelofMVDinSCCgroupwassignificantlyhigherthanthatinBowendiseasegroup（P<0.05）,andthelatterwassignificantlyhigherthanthatinnormalskingroup（P<0.05）.CONCLUSION：MMP9ishighlyexpressedinSCC,andmaybeoneofimportantfactorsinangiogenesisofSCC.

【Keywords】skinneoplasms;carcinoma,squamouscellimmunohistochemistry;gelatinaseB;antigens,CD34;microvesseldensity

【摘要】目的：探讨皮肤鳞状细胞癌（SCC）发生发展过程中基质金属蛋白酶9（MMP9）对微血管生成的意义.方法：SCC45例,Bowen病19例、正常皮肤10例进行CD34,MMP9免疫组化染色.检测MMP9的表达和微血管密度（MVD）的变化，分析MMP9的表达与MVD之间，及它们与SCC临床病理特征之间的关系.结果：在SCC组中MMP9表达高于Bowen病组（P<0.05）,Bowen病组中MMP9表达高于正常皮肤组（P=0.028,P<0.05）.在SCC组中MVD值高于Bowen病组(P<0.05)，Bowen病组中MVD值高于正常皮肤组（P<0.05）.结论：MMP9可能是SCC促进微血管生成因子之一，与SCC的侵袭性相关.

【关键词】皮肤肿瘤；免疫组织化学；明胶酶B；抗原CD34；微血管密度